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A Quick Dive Into Ketones

Will going “keto” i.e. eating a low-carb, high-fat diet, help you lose weight, boost your endurance and most importantly protect you from CNS oxygen toxicity on those long, deep scooter dives? (We’re talking about you Karst Underwater Research) Former WKPP member, now a human performance research scientist, Dawn Kernagis summarizes the state of the science. Call it Keto Rising!

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by Dawn Kernagis, PhD

If you follow the current trends in health and fitness, the words; ‘ketone’, ‘ketogenic’, and ‘ketosis’ have recently become synonymous with weight loss, wellness, endurance, and (of course) controversy with respect to effectiveness and safety. Despite the popularity of these terms, there is still a bit of confusion surrounding each of them and, specific to the undersea community, what research has been conducted on ketones and diving. 

In this post, I will provide a brief, high-level overview of what ketosis is, what we know about potential diving-related applications of ketones, and next steps in diving-related ketosis research.

Keto 101

Ketosis refers to the presence of elevated ketone bodies (also known as ketones)  in the blood. Ketones (beta-hydroxybutyrate,  acetoacetate, and acetone) are produced by the liver from stored fat when a person either strictly fasts (typically a minimum of 24-48 hours) or undertakes a ‘ketogenic diet’ (very low carbohydrate, low-moderate protein, high fat). During ketosis, the body shifts from using glucose as a fuel, as a result of carbohydrate restriction, to burning ketone bodies.

In addition to fasting and the ketogenic diet (endogenous approaches), ketosis is also achievable exogenously by consuming substances such as ketone salts or ketone esters. Exogenous ketones increase blood ketone levels to varying degrees, with the latter raising blood ketone levels greater than 4mmol/liter  without depletion of carbohydrate stores, even in the presence of a carbohydrate-rich meal (Stubbs 2017). Induction of ketosis by eating or drinking ketones is rapid and acute; that is, the ketones will typically be metabolized within a maximum of four to eight hours. Given their short-lived effects, research is underway to understand if regular consumption of exogenous ketones has the same effect on our cells, tissues, and overall physiology as a sustained keto diet or fasting. 

Ketosis can be monitored using a variety of commercially available options, including sampling blood from the fingertip (similar to blood glucose testing) to measuring blood ketones with a handheld meter, using urine test trips, and analyzing exhaled breath. The ketogenic diet is actually one of the few diets that has a biomarker that tells you whether or not you are actually ‘on’ the diet: if your ketone levels are above the threshold, you are in the physiological state of ketosis, and vice versa! 

The number of studies assessing the benefits versus potential risks of sustained ketosis are increasing, and the medical and sports science communities are grasping an improved understanding about if, when, and how a ketogenic diet or exogenous ketone consumption could either benefit or negatively impact health and performance. While summarizing these studies would be the subject of an extensive series of blog posts, the study that piqued the diving community’s interest in ketones was focused on protecting rats from CNS oxygen toxicity seizures.

Bringing Ketones into Diving

The history of ketosis in the medical community is largely centered around epilepsy treatment. While there are historic records of the starvation ketosis being used as a treatment approach going back to 400 B.C., the first formal clinical trials using dietary ketosis to treat epilepsy were conducted in the 1920s. Dr. Russell Morse Wilder at the Mayo Clinic developed the ketogenic diet as a way to obtain the benefits of fasting through a nutritional approach that could be sustained long-term. In 1921, he conducted the first trial to assess the effect of a ketogenic diet on epilepsy patients.

Photo courtesy of Dawn Kernagis.

Flash forward to just a decade ago, when Dr. Dominic D’Agostino at University of South Florida became interested in the effects of ketosis on seizure latency and its potential as a mitigation strategy for CNS oxygen toxicity in divers. With previous experience conducting research in undersea medicine, Dr. D’Agostino developed a study to assess the effects of exogenous ketosis (induced by consumption of a novel ketone ester, known as an acetoacetate diester) on oxygen toxicity seizure latency in small animals (D’Agostino 2013). Rats were monitored for oxygen toxicity symptoms and brain activity using EEG during a 5 ATA chamber dive on 100% oxygen, and they were either dosed with ketone diester (which exogenously increases all three ketone bodies), butanediol (which only increases the ketone body beta-hydroxybutyrate),  or water (control) prior to their dive. 

Compared to water, the ketone diester induced a rapid and sustained elevation of all three ketone bodies and increased latency by a factor of five compared with water. Butanediol did not increase seizure latency even though it did effectively elevate blood beta-hydroxybutyrate. Demonstration of the increased oxygen toxicity seizure latency in rats in response to the ketone bodies acetoacetate and acetone, but not beta-hydroxybutyrate, was a significant finding. The study results also point to the different effects of the three different ketone bodies and the fact that not all exogenous ketones are the same when it comes to their potential applications. 

It is important to note that findings from animal studies can fail to translate effectively to humans due to differences in physiology and a variety of additional factors. A human study that assesses the effects of the ketone diester on CNS oxygen toxicity will be critical before potential translation to the diving community. However, controlled human studies of human oxygen toxicity are also rare and can be difficult to conduct. 

Given the low incidence of oxygen toxicity in divers, along with the ethical considerations of conducting oxygen toxicity interventional research in a non-medical, field-based setting, these human studies need to be conducted in a laboratory environment with the appropriate safety considerations, statistically-driven study design, and outcome measurements. As a starting point, and at the time of this article, a double-blinded, lab-based study of oxygen toxicity in humans on a ketogenic diet compared to normal diet was underway at Duke University.

Future Research

When it comes to ketosis, there is still a significant amount of research that needs to be conducted before we know whether it could be beneficial and, most importantly, reliably safe in divers in a variety of conditions and exposures. In addition to the Duke oxygen toxicity study in humans, there are laboratory studies currently underway to evaluate the effect of ketone esters on cold water performance (e.g., core temperature, hand temperature, dexterity, and diver performance), hypoxia mitigation, and a variety of physical performance parameters. Beyond diving-specific applications, long-range studies that assess the health effects of sustained ketosis are underway and will provide a better understanding as to how the ketogenic diet and repetitive dosing of exogenous ketones impact long-term health and performance.


Dr. Dawn Kernagis is a research scientist in the area of human performance optimization and risk mitigation for operators in extreme environments, such as those working undersea, at altitude, and in space. Her team’s current research efforts are funded through several DoD agencies and the NASA Translational Institute for Space Health.

Dawn came to IHMC from Duke University Medical Center, where she was an American Heart Association Postdoctoral Fellow and funded by the Office of Naval Research (ONR) to identify mechanisms and potential therapeutic targets for acute brain injury. She completed her PhD at Duke University as ONR Undersea Medicine’s first Predoctoral Award recipient. 

Dawn is a technical diver and has managed numerous underwater exploration, research, and conservation projects around the world since 1993, including the deep underwater exploration of Wakulla Springs and surrounding caves. Dawn was inducted into the Women Divers Hall of Fame in 2016, and is a Fellow of The Explorers Club. In 2018, she received the Undersea and Hyperbaric Medical Society’s Young Scientist Award. She loves traveling, playing piano, and spending as much time outdoors and on the water as possible.

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Part Two: “Tech Divers, Deep Stops, and the Coming Apocalypse”

In part two of this four-part series on the history and development of GUE’s decompression protocols, GUE founder and president Jarrod Jablonski discusses the lack of appropriate mixed gas decompression tables in the early days of technical diving. He goes on to discuss the initial development of “ratio decompression” and the early thinking and rapid adoption of “deep stops,” which have come under pressure as a result of new research that questions their efficacy and safety. He also discusses the emergence of the now nearly ubiquitous gradient factors (GF) used in most dive computing. Feel free to DIVE IN and share your thoughts.

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If you have not read Part One of the “Decompression, Deep Stops, and the Pursuit of Precision in a Complex World” series please find it here.

by Jarrod Jablonski

Header Photo by JP Bresser

Technical diving means different things to different people, opening legitimate arguments about different time periods and personalities involved in shaping this activity. Our purpose is more confined as I wish to focus mainly upon the development of decompression procedures. Leading figures like Jacques Cousteau (1910–1997) performed reasonably significant “technical” dives including both deep and overhead exposures. Yet, his dives were relatively short, often on air, and absent a community of fellow tech divers with whom to evolve varying strategies. Meanwhile, military or commercial activities reached significant depth with notable exposures but used different technologies and procedures. Hopefully, the following overview will be independently interesting—although I also hope to illustrate what I consider an intriguing wrinkle currently lacking in most debates over the best ascent schedule for a decompression dive.

For our purposes, I mark the 1980s as the period in which technical diving truly started to become a globally recognized activity. Of course, numerous significant projects occurred before this period, but the 1980s established a growing global awareness that amazing diving feats were possible by enthusiasts. The 1990s and the developing internet age brought these ideas progressively into the mainstream. Most importantly, these global communities could now easily communicate information and, of course, disagree about the best way to do one thing or another. 

Jarrod Jablonski, George Irvine, and Brent Scarabin preparing for dives in Wakulla Springs (late 1990s). Photo from the GUE archives.

The 1980s and 1990s were also an interesting period of development because people were doing more aggressive dives but still lacked important support tools that would take another decade to materialize. For example, most early tech dives were using some form of military, commercial, or scientific tables, such as the U.S. Navy, Oceaneering International, Inc. or the National Oceanic and Atmospheric Administration (NOAA), which were often not well-suited to the dive at hand. This is because there were no decompression programs available. Divers were unable to calculate their own profiles and would trade whatever tables they could gather. In many cases, they were forced to choose from tables that were calculated at a different depth, time, and/or breathing mix to the planned dive. 

George Irvine after a dive with the WKPP. Photo from the GUE archives.

Over time, the physiologist Dr. R.W. “Bill” Hamilton (1930–2011) began creating custom tables, but the lack of flexibility, as well as the cost, discouraged frequent use for most divers, especially when considering a range of depth profiles and their various time adjustments. This problem was especially prominent in deep cave diving where variable profiles and long bottom times created numerous complications . This landscape encouraged, if not demanded, that exploration divers be creative with their decompression practices. For example, Woodville Karst Plain Project (WKPP) explorers George Irvine and I began to explore ways to extrapolate from existing tables. The birth of what is now called ratio decompression originated with this practice. 

By exploring the way decompression schedules develop, we began outlining simple ratios that allowed divers to adjust their decompression based upon a ratio of time spent at depth. Then, in 1998, I went on to form Global Underwater Explorers (GUE), and such practices became part of the process for helping divers appreciate the structure of decompression while supporting adjustments to profiles when depth or time varied from that expected. Regrettably, some individuals took this too far and began promoting complex adjustments and marketed them as superior to the underlying algorithms from which they had been derived.

We should leave a more in-depth review of ratio deco for another time. For now, I intend to illustrate that “rules” such as ratio deco had their roots in limited availability of decompression tables and evolved as a useful tool for understanding and estimating decompression obligation. The fact that early tech divers had limited ability to calculate profiles encouraged a “test-and-see” philosophy, further fueling the early popularity of ad hoc adjustments to decompression profiles. This was most notable in the DIR and GUE communities through ratio-style adjustments and also prominent with non-DIR advocates who used a modification known as Pyle-stops, originating from ichthyologist Richard Pyle’s early deep dives and his attempts to refine efficient ascent protocols. One common adjustment in these approaches involved a notable reduction in ascent speed, adding additional stops known as “ deep stops

Driven by self-discovery, the migration toward deep stops resulted in rare agreement among technical divers, and the practice developed a life of its own in conference symposiums, being advocated far and wide by a healthy share of technical divers. The earliest phases of these modifications were driven in part by limited access to decompression tables, though by the end of the 1990s there were a variety of decompression programs available. This was a big improvement, although it was still true that divers had a limited baseline of successful dives while planning mixed-gas dives in the 50 to 100 meter range and beyond. Today, most divers take for granted that technical dives planned with available resources and within today’s common use (a few hours around 90m/300 ft) are relatively safe in terms of decompression risk. When these programs first came to market, there remained many question marks about their efficacy. 

Jarrod Jablonski and Todd Kincaid use Doppler while evaluating decompression profiles in 1995. Photo from the GUE archives.

The availability of decompression programs was a big advantage for technical divers, as they now had more sophisticated tools at their disposal. Yet, the output from many of these programs produced tables that were sometimes different to profiles thought to be successful by some groups. Typically, the difference related to an increase in the total decompression time and/or a distribution of stops that varied from developing consensus. These divers were keenly interested in the developing tools but reluctant to change from what appeared successful, especially when that change required additional hours in the water. A debate developed around the reason for these differences, bringing the already brewing interest in bubbles well into the mainstream. 

At the time, all tables were based upon dissolved gas models like Buhlmann, and thus not directly modeling bubble development during an ascent. Dissolved gas models preference ascents that maintain a reasonably high gradient between the gas in tissues and the gas being breathed, which should be supportive of efficient elimination from tissues. Dissolved gas models manage—but do not explicitly control for—bubbles and were thus labeled (probably unfairly) as “bend and mend” tables. In fact, dissolved gas models are designed to limit supersaturation (explicitly), because this is supposed to limit bubble formation. Haldane references this control of supersaturation in his pioneering publication .

Those advocating for different ascent protocols imagine that a lack of deep stops creates more bubbles, which then need to be managed during a longer series of shallow decompression stops. In this scenario, a slower ascent from depth, including “deep stops,” would reduce the formation and growth of bubbles while reducing time that might otherwise be needed to manage previously formed bubbles. Support for these ideas gained momentum in diving and professional communities, although some of these individuals were arguably conflicted by a vested interest in promoting deep stop models. Eventually, the practice received more critical consideration but during the intervening years deep stops were largely considered as common knowledge.

Jarrod Jablonski and George Irvine at the last stop of a 15-hour decompression in 1998. Photo from the GUE archives.

The idea of controlling bubbles became extremely popular through the 1990s, encouraging deeper stops designed to limit bubble formation and growth. The “test-and-see” approach developed by early tech divers appears to have fueled the promulgation of deep stops though the determining characteristics remained poorly defined. Early tech divers embraced the uncertainty of their activity, realized that nobody had the answers, and decided to “experiment” in search of their own answers. In fact, divers were experimenting with a lot more than deep stops, altering gases breathed, the placement of various decompression stops, and the total amount of decompression time utilized. This meant that divers were sometimes aggressively adjusting multiple factors simultaneously. 

For example, divers using a decompression program might input significantly less helium than was present in their breathing mixture. This was done because the algorithm increased decompression time with elevated helium percentage, sometimes known as the helium penalty . In other cases, divers would completely change the structure of stop times. For example, divers would invert the way a profile should be conducted by doing more time near a gas switch and less time prior to the next gas switch, believing the higher-oxygen gas only 3m/10ft away was time better utilized. 

I do not intend an exhaustive or detailed review but only to assert the many, sometimes radical approaches being taken by tech divers who often perceived success with these various strategies. In some cases, it appears these practices may have been leading the way toward improvements (eliminating the Helium penalty) while other adjustments might prove disadvantageous. In both cases, it is important to note that divers understood—or should have understood—these actions to be potentially dangerous, accepting risk as a natural part of pushing into uncertain territory where definitive guidance and clear borders are rarely available. 

None of this is to argue that divers should engage in aggressive decompression or challenge convention or place themselves at risk to unknown complications. I merely wish to clarify the atmosphere under which these adjustments were conducted, while highlighting that conventional ideas of risk mitigation are inherently complicated against a backdrop of novel exploration. A sense of relative risk dominated most of these trials since there are also risks associated with lengthy, in-water exposure. Eliminating what might be an avoidable decompression obligation could reduce risk from other obvious factors, including changing weather, dangerous marine life, being lost at sea, hypothermia, and oxygen exposure. Decompression experimentation was but one of many attempts to establish new protocols during extensive exploration projects. 

During this time, deep stops and similar adjustments were part of the “norm” for aggressive technical explorers who were sometimes notably reducing in-water time as compared to available tables. It is interesting to note that individual differences in susceptibility seemed among the most prominent variables across the range of tested adjustments, and we will return to this in a later discussion. For now, we should acknowledge that the “success” being achieved (or imagined) is greatly complicated by a small sample size of self-selected individuals who were simultaneously experimenting with a range of variables. On the other side, I do not want to entirely discount the results being seen by these divers. We should remember that development of safe decompression ascents for the general diving community was not the goal of most tech divers. These divers were interested in maximizing their personal and team efficiency during decompression. These strategies may or may not have been objectively successful or broadly applicable, but many teams imagined them so, at least within the narrow scope being considered. Later, we will return to these important distinctions with careful consideration for the potentially different interests being pursued by divers evaluating different decompression profiles.

Fortunately, a desire to create broadly useful tools was high on the list of priorities for some individuals in the technical diving community, leading to a relevant and important contribution. This inventive approach would introduce a new way to think about decompression, remaining to this day at the center of the debate about deep stops. 

Creating a New Baseline

Despite the popularity of deep stops and other modifications presented previously, technical divers lacked a common language for comparing the results, especially across different profiles. Compounding this problem was the variable way decompression programs considered a dive to be more or less “safe.” For example, some programs developed “safety factors” which increased total decompression time by an arbitrary factor, i.e., made them 10% longer. Other programs used different strategies, though it was not clear whether any of the various safety factors actually made the decompression safer. Whether safer or not, these factors were typically inconsistent and added complications when comparing various profiles. Just as these debates were reaching a fervor, help arrived from an unlikely place. An engineer by trade and decompression enthusiast by choice, Erik Baker had developed a novel solution. 

George Irvine passing time during a long decompression. Photo from the GUE archives.

Baker was seeking a way to establish consistency in considering the safety or lack of safety between various profiles. The term Baker applied was Gradient Factor (GF). I will not invest considerable time exploring the science behind GF, as many useful resources are widely available. For our purposes, it is sufficient to say that GFs allows a user to establish a lower threshold than the maximum recommended by a dissolved gas algorithm. This maximum pressure or M-value is assigned to “compartments” having an assumed amount of tolerance relative to the flow of blood they receive. Decompression theory is complicated by many factors but when the pressure of a gas in a compartment nears the M-value, it is thought that the risk of decompression sickness becomes higher. By adjusting a profile through the use of GF, one presumably reduces the risk. However, this also means there is less gradient between the gas in tissues and the gas in blood, reducing the driving force for the removal of gas and probably requiring additional decompression time. 

Gradient factors took an important step toward using a consistent language when talking about a variety of adjustments divers might make to their decompression. As part of his work on GF, Baker had developed a keen interest in the decompression adjustments by leading technical divers. Baker and I began working together while evaluating some of our most extreme diving profiles. This collaboration led to a number of productive developments including GUE’s DecoPlanner, released in 1997, and among the first to utilize GF methodology. These collaborations further highlighted what appeared to be a discrepancy between the decompression expected by dissolved gas algorithms and the decompressions being conducted by many technical divers. 

Decompression experimentation tended to cluster in small groups whose size was likely affected by self-selection with members who would stop or reduce aggressive dives when experiencing decompression sickness. Yet, it seemed possible that there was more to the story. These divers were doing several things that should have exposed them to notable risk and yet were repeatedly completing decompressions of more than 15 hours. Deep stops were only part of the story as these divers were ignoring conventional wisdom in several areas while notably reducing decompression time. What was behind this discrepancy? Were deep stops right or wrong? Was the conservative approach to helium right or wrong? Were these individuals lucky? Were they unusually resistant to decompression sickness, or were there other factors lurking in the background?

Note: I will outline many of these developments in the upcoming Part Three, where we more directly consider the modern challenges to deep stops and most especially the assertion they are dangerous. In the interim, I hope to hear from our readers. Do you have different experiences from this period? Do you think such experimentation is reckless or inadvisable? Please let us know your thoughts.


Jarrod is an avid explorer, researcher, author, and instructor who teaches and dives in oceans and caves around the world. Trained as a geologist, Jarrod is the founder and president of GUE and CEO of Halcyon and Extreme Exposure while remaining active in conservation, exploration, and filming projects worldwide. His explorations regularly place him in the most remote locations in the world, including numerous world record cave dives with total immersions near 30 hours. Jarrod is also an author with dozens of publications, including three books.

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In this video, GUE President Jarrod Jablonski and Technical Administrator Richard Lundgren discuss some of the early hurdles associated with decompressing from deep tech and cave dives. This discussion explores the lack of readily available deep-diving tables and most especially those capable of managing multi-level profiles. They also explore the early development of ratio deco and similar “experiments” conducted by early technical divers.

What is Ratio Deco?

The basic idea of ratio deco involves establishing a ratio between the time spent at a given depth and the associated decompression. This is possible because the curve describing the relationship between bottom time and total stop time (TST) at a single depth can be approximated by a straight line. The straight-line approximation breaks down beyond a certain range but can be useful toward estimating decompression time. For example, the ratio in a typical tech dive at 45 m/ 150 ft using the appropriate gases is 1:1, meaning that a dive at 45 m/150 ft for 30 min will result in a decompression of 30 min while assuming appropriate bottom and decompression gasses.

We can also follow with adjustments for deeper dives so that each 3 m/10 ft deeper than planned would result in a decompression extension of five minutes. At some point the increase or decrease in the baseline parameter will break down, leading to profiles that are too conservative or too liberal and we need a new ratio such as the GUE standard for 75 m/250 ft at 2:1 where 30 minutes of bottom time results in 60 minutes of decompression, assuming appropriate decompression gasses arranged through a properly staged ascent.

What exactly are deep stops?

Deep stops are technically any stops added below what would otherwise be established by a typical dissolved gas model. Dissolved gas models establish faster ascents when compared to models that intend to reduce development of bubbles. Bubble-oriented models seek to reduce bubble development by ascending more slowly, usually by adding pauses or "deep stops".

“The formation of gas bubbles in the living body during or shortly
after decompression evidently depends on the fact that the partial
pressure of the gas or gases dissolved in the blood and tissues is in
excess of the external pressure. But it is a well-known fact that
liquids, and especially albuminous liquids such as blood, will hold gas
for long periods in a state of supersaturation, provided the super
saturation does not exceed a certain limit. In order to decompress
safely it is evidently necessary to prevent this limit being exceeded
before the end of decompression.”

Boycott AE, Damant GCC, Haldane JS. The prevention of compressed-air illness. J Hygiene (Lond ) 1908;8:342-443.

The extra decompression time calculated by various algorithms when breathing a helium mix is a consequence of the long held belief that helium, which is lighter than air, enjoys faster uptake by the body than nitrogen (in the case of the Buhlmann algorithm 2.65 times faster).

Compartments are hypothetical tissues which are intended to model how gas moves in and out of tissues where blood flow varies. Fast compartments are intended to model movement in a tissue with a lot of blood flow and slow compartments strive to model behavior in tissues with very little blood flow.